gamma-Aminobutyric acid responses in rat locus coeruleus neurones in vitro: a current-clamp and voltage-clamp study.
نویسندگان
چکیده
منابع مشابه
Automated voltage-clamp technique.
The voltage-clamp electrophysiology method is the gold standard for measuring the function of ion channels. In the past, this technique has had limited applicability in pharmaceutical drug discovery because of its low throughput, steep learning curve, and challenges in standardization of the experiments. Recently, new electrophysiology platforms have been developed, which are based on the use o...
متن کاملVoltage clamp techniques
Much of what we know about the properties of ion channels in cell membranes has come from experiments using voltage clamp. In general, the method allows ion flow across a cell membrane to be measured as electric current, whilst the membrane voltage is held under experimental control with a feedback amplifier. The method was first developed by Cole (1949) and Hodgkin et al. (1952) for use with t...
متن کاملVoltage and current clamp transients with membrane dielectric loss.
Transient responses of a space-clamped squid axon membrane to step changes of voltage or current are often approximated by exponential functions of time, corresponding to a series resistance and a membrane capacity of 1.0 muF/cm(2). Curtis and Cole (1938, J. Gen. Physiol.21:757) found, however, that the membrane had a constant phase angle impedance z = z(1)(jomegatau)(-alpha), with a mean alpha...
متن کاملA 5-hydroxytryptamine2 agonist augments gamma-aminobutyric acid and excitatory amino acid inputs to noradrenergic locus coeruleus neurons.
We examined the effects of the 5-hydroxytryptamine2 receptor agonist, (+-)1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane, on spontaneous and evoked discharge of locus coeruleus neurons in the rat. Extracellular recordings were obtained from single locus coeruleus neurons while (+-)1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane was injected systemically or locally into the locus coeruleus. Systemi...
متن کاملStudies on Calcium and Sodium in Uterine Smooth Muscle Excitation under Current-Clamp and Voltage-Clamp Conditions
The objective of these studies was to define the roles of calcium and sodium in uterine smooth muscle excitation. The double sucrose-gap technique was used for current-clamp and voltage-clamp experiments. It was shown that neither sodium nor calcium alone is capable of supporting excitation in estrogen-dominated uterine smooth muscle. Calcium dependence was explained in part by increased membra...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1990
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1990.sp017938